| State | County | Location | mi[1][2] | km | Old exit | New exit | Destinations | Notes |
|---|---|---|---|---|---|---|---|---|
| Florida | Hillsborough | Tampa | 0.000 | 0.000 | — | — |   I-275 (SR 93) – St. Petersburg, Ocala, Tampa International Airport | Westbound splits into 3 ramps: Northbound & southbound I-275 and "Exit 45A" (in reference to the I-275 exit number)/"Downtown East-West", which joins the southbound I-275 Exit 45A ramp without joining the southbound I-275 mainline. |
| 1.154 | 1.857 | — | 1 | 22nd Street / 21st Street – Cruise Ships, Ybor City National Historic Landmark District | former SR 585 | |||
| 1.76 | 2.83 | — | 2 |    SR 618 (Selmon Expressway) / US 41 Bus. (20th Street) – Brandon, Port Tampa Bay | I-4–Selmon Expressway Connector (left exits, both directions); access to or from SR 618 only in the same direction; US 41 Bus. (trucks only, signed only as "Port Tampa Bay") access from westbound I-4 and to eastbound I-4 | |||
| 2.463 | 3.964 | — | 2 |  SR 569 (40th Street) | Former interchange | |||
| 3.266 | 5.256 | — | 3 | US 41 (50th Street / SR 599) / Columbus Drive | Eastbound left exit and westbound left entrance; eastbound right entrance and westbound left entrance | |||
| | 4.706 | 7.574 | 4 | 5 |  SR 574 (Martin Luther King Jr. Boulevard) | |||
| 5.573 | 8.969 | 5 | 6 | Orient Road | Eastbound exit and westbound entrance | |||
| 6.683 | 10.755 | 6 | 7 |   US 92 (Hillsborough Avenue / SR 600) / US 301 (SR 41) – Riverview, Zephyrhills, Busch Gardens, Tampa Executive Airport, State Fairgrounds | Access to/from US 92 east eastbound; Access to/from US 92 west westbound; Access to US 301 both directions | |||
| 8.610 | 13.856 | 7 | 9 |  I-75 (SR 93A) – Ocala, Naples, Brandon, Temple Terrace | I-75 exit 261 | |||
| Mango | 10.142 | 16.322 | 8 | 10 |  CR 579 (Mango Road) – Mango, Thonotosassa | |||
| Dover | 13.876 | 22.331 | 9 | 14 | McIntosh Road – Dover | |||
| 17.434 | 28.057 | 10 | 17 | Branch Forbes Road – Dover | Serves Dinosaur World | |||
| Plant City | 19.518 | 31.411 | 11 | 19 |  SR 566 (Thonotosassa Road) | |||
| 21.280 | 34.247 | 13 | 21 |    SR 39 (Alexander Street) / CR 39 (Buchman Highway) | Alexander Street was originally old exit 12, but was combined with 13; access to South Florida Baptist Hospital | |||
| 22.596 | 36.365 | 14 | 22 | Park Road (SR 553) | SR 553 not signed | |||
| Hillsborough–Polk county line | Lakeland | 25.563 | 41.140 | 15 | 25 | County Line Road | ||
| Polk | 26.530 | 42.696 | 15A | 27 |   SR 570 east (Polk Parkway) – Lakeland, Winter Haven, Bartow | |||
| 28.365 | 45.649 | 16 | 28 |  To US 92 (SR 546/Memorial Boulevard) – Lakeland | SR 546 not signed | |||
| 30.675 | 49.367 | 17 | 31 |  SR 539 (CR 35A north/Kathleen Road) – Kathleen, Lakeland | ||||
| 32.003 | 51.504 | 18 | 32 |  US 98 (SR 35 / SR 700) – Lakeland, Dade City | ||||
| 33.440 | 53.816 | 19 | 33 |   SR 33 (Lakeland Hills Boulevard) / CR 582 (Socrum Loop Road) – Lakeland | Eastbound exit/entrance with SR 33 only (signed "SR 33"); westbound exit/entrance with CR 582 only (signed "CR 582"/"To SR 33") | |||
| 37.894 | 60.984 | 20 | 38 | SR 33 | ||||
| 41.223 | 66.342 | 20A | 41 |  SR 570 west (Polk Parkway) – Auburndale, Lakeland | SR 570 exit 24 links to I-4 eastbound; Serves Florida Polytechnic University (southwest corner of interchange). | |||
| | 43.981 | 70.781 | 21 | 44 |  SR 559 – Polk City, Auburndale | Serves Fantasy of Flight | ||
| 47.982 | 77.220 | 22 | 48 |  CR 557 (Old Grade Road) – Lake Alfred, Winter Haven | ||||
| Four Corners | 54.733 | 88.084 | 23 | 55 |  US 27 (SR 25) – Haines City, Clermont | Serves Legoland and Heart of Florida Regional Medical Center | ||
| Osceola | 57.723 | 92.896 | 24 | 58 |  CR 532 (Champions Gate Boulevard/Osceola-Polk Line Road) – Poinciana, Kissimmee | |||
| 59.663 | 96.018 | — | 60 |   SR 429 north (Western Expressway) – Apopka | SR 429 exit 1 links to I-4 eastbound | |||
| Celebration | 61.781 | 99.427 | 24C-D-E | 62 |  SR 417 north (Central Florida GreeneWay) – Disney World, Celebration, Orlando International Airport, Sanford | Collector/distributor lanes serve two junctions with one exit. Full interchange with World Drive. Eastbound exit to SR 417. Westbound entrance from SR 417. | ||
| 64.165 | 103.264 | 25A-B | 64 |  US 192 (SR 530/Irlo Bronson Memorial Highway) – Kissimmee, Magic Kingdom, Hollywood Studios | Access to AdventHealth Celebration | |||
| | 65.322 | 105.126 | 26C-D | 65 |  Osceola Parkway (CR 522) – Animal Kingdom, Wide World of Sports | |||
| Orange | Lake Buena Vista | 66.565 | 107.126 | 26A-B | 67 |  SR 536 east (Epcot Center Drive/World Center Drive) to SR 417 north – Epcot, Disney Springs | ||
| 68.107 | 109.608 | 27 | 68 |  SR 535 – Kissimmee, Lake Buena Vista | ||||
| Williamsburg | 70.983 | 114.236 | 27A | 71 | Sea World (Central Florida Parkway) | Eastbound exit and westbound entrance | ||
| 71.744 | 115.461 | 28 | 72 |  SR 528 east (Beachline Expressway) – Orlando International Airport, Cape Canaveral | ||||
| Orlando | 73.732 | 118.660 | 29A | 74A |  SR 482 (Sand Lake Road) / International Drive | |||
| 75.246 | 121.097 | 29B | 74B | Universal (Adventure Way) | Westbound exit and entrance (other movements via exit 75A) | |||
| 75.246 | 121.097 | 30 | 75 |  SR 435 (Kirkman Road) / International Drive – Universal | Signed as exits 75A (south) and 75B (north); serves Volcano Bay and Fun Spot America | |||
| 76.359 | 122.888 | 31 | 77 |  Florida's Turnpike (SR 91) – Miami, Ocala | Turnpike exit 259 | |||
| 77.760 | 125.143 | 31A | 78 | Conroy Road | Serves The Mall at Millenia, Holy Land Experience | |||
| 79.147 | 127.375 | 32 | 79 |  CR 423 (John Young Parkway) | ||||
| 80.474 | 129.510 | 33 | 80 |   US 17 / US 92 / US 441 (Orange Blossom Trail / SR 500 / SR 600) | Signed as exits 80A (south/west) and 80B (north/east) eastbound; previously signed as exits 33A and 33B accordingly; westbound is a left exit, signed as exit 80 and only providing south/west access | |||
| 81.004 | 130.363 | 34 and 81A | 81 | To US 17 north / US 92 east / US 441 north / Michigan Street | Westbound exit and eastbound entrance | |||
| 81.469 | 131.112 | 35 | 81BC | Kaley Avenue | Eastbound exit and entrance; westbound ramps closed in 2015; access to Orlando Regional Medical Center, Arnold Palmer Hospital for Children, and Winnie Palmer Hospital for Women & Babies | |||
| 82.116 | 132.153 | 36 | 82A |  SR 408 (East–West Expressway) | ||||
| 82.235 | 132.344 | 37 | 82B | Gore Street | Former westbound-only interchange; westbound entrance remains | |||
| 82.636 | 132.990 | 38 | 82B | Anderson Street / South Street | South Street signed eastbound; Anderson Street signed westbound; To Amway Center, History Center; formerly signed as exit 82C | |||
| 82.78 | 133.22 | 39 | 83 | South Street | Closed interchange; no eastbound exit | |||
| 83.30 | 134.06 | 40 | 83A |  SR 526 (Robinson Street) | Former interchange; eastbound exit and westbound entrance | |||
| 83.792 | 134.850 | 41 | 83A |  US 17 / US 92 / SR 50 (Colonial Drive / SR 600) / Amelia Street – Bob Carr | Signed as exit 83A westbound; formerly signed as exit 83B eastbound | |||
| 84.279 | 135.634 | 42 | 84 | Ivanhoe Boulevard | Westbound exit (signed as exit 84B westbound), eastbound entrance; eastbound exit closed in 2015 | |||
| 85.135 | 137.012 | 43 | 85 | Princeton Street | Access to AdventHealth Orlando | |||
| 85.890 | 138.227 | 44 | 86 | Par Street | Eastbound exit and westbound entrance | |||
| Winter Park | 86.789 | 139.673 | 45 | 87 | Fairbanks Avenue (SR 426) | Temporarily moved to miles back from original spot, merging with exit 86-Par Street | ||
| 87.767 | 141.247 | 46 | 88 |  SR 423 (Lee Road / US 17 Truck south / US 92 Truck west) | Western end of US 17 Truck / US 92 Truck overlap; serves Eatonville | |||
| Maitland | 89.491 | 144.022 | 47 | 90 |  SR 414 (Maitland Boulevard / US 17 Truck north / US 92 Truck east) | Eastern end of US 17 Truck / US 92 Truck overlap; signed as exits 90A (east) and 90B (west) | ||
| Seminole | Altamonte Springs | 91.631 | 147.466 | 48 | 92 |  SR 436 – Altamonte Springs, Apopka | Access to AdventHealth Altamonte | |
| Longwood | 93.613 | 150.656 | 49 | 94 |  SR 434 – Longwood, Winter Springs | |||
| Lake Mary | 98.400 | 158.359 | 50 | 98 | Lake Mary, Heathrow, Orlando Sanford International Airport (Lake Mary Boulevard) | |||
| 100.628 | 161.945 | 51A | 101A |  CR 46A – Sanford, Heathrow | ||||
| Sanford | 101.366 | 163.133 | — | 101B |  SR 417 south (Seminole Expressway) – International Airports (Sanford, Orlando), Sanford | |||
| 102.505 | 164.966 | 51 | 101C |  SR 46 – Mount Dora, Sanford Historic District | ||||
| | 103.997 | 167.367 | 52 | 104 | US 17 / US 92 (SR 15 / SR 600) – Sanford | Access to Central Florida Regional Hospital | ||
| St. Johns River (Lake Monroe) | St. Johns River Veterans Memorial Bridge | |||||||
| Volusia | Deltona–DeBary city line | 107.821 | 173.521 | 53 | 108 | DeBary, Deltona (CR 4162) | ||
| Deltona–Orange City city line | 110.636 | 178.051 | 53C | 111 | Deltona, Orange City, DeBary (CR 4146) | Signed as exits 111A (Deltona) and 111B (Orange City, DeBary); previously signed as exits 53CA and 53CB accordingly; access to AdventHealth Fish Memorial | ||
| Deltona–DeLand city line | 113.783 | 183.116 | 54 | 114 |  SR 472 – Deltona, DeLand | |||
| Lake Helen | 115.898 | 186.520 | 55 | 116 | DeLand, Lake Helen Historic District (CR 4116) | |||
| DeLand | 118.456 | 190.636 | 56 | 118 |  SR 44 – New Smyrna Beach, DeLand Historic District | Signed as exits 118A (east) and 118B (west) | ||
| Daytona Beach | 129.131 | 207.816 | 57 | 129 | US 92 east (SR 600) – Daytona Beach | Eastbound exit and westbound entrance; access to Daytona Beach International Airport and Halifax Health Medical Center | ||
| 131.987 | 212.412 | — | 132 |  I-95 (SR 9) – Daytona Beach, Jacksonville, Miami | I-95 exit 260B; signed as exits 132A (north) and 132B (south) | |||
| 132.298 | 212.913 | 58, 132 | — |  SR 400 east – South Daytona | ||||
1.000 mi = 1.609 km; 1.000 km = 0.621 mi
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- ^ Florida Department of Transportation (August 4, 2010). "Florida Department of Transportation Interchange Report" (PDF). Florida Department of Transportation. pp. 2–3. Archived from the original (PDF) on February 8, 2012. Retrieved November 2, 2010.
- ^ Florida Department of Transportation. "Straight Line Diagrams". Florida Department of Transportation. Archived from the original on March 6, 2014. Retrieved March 8, 2014.