Neildsilva

Gp41 Article Peer Review Edits by Monica P.:

• Why is HIV italicized in intro?
• "Gene and post-transnational modifications" Title is not HS level and not explained
• Structure is too HIV specific; article is not on Gp41 HIV but just Gp41
• Insert comma: "The core of gp41 folds into a six helical bundle structure exposing the previously hidden gp41 fusion peptides(,) which then assist in the fusion with the host cell."
• Insert comma and s: "The activation process occurs readily(,) which suggest(s) that the inactive state of gp41 is metastable and the conformational changes allow gp41 to achieve its more stable active state."
• "As a drug target" connect section to more gp41
• "The pre-hairpin structure has a relatively long half-life which makes it a (potential) target for therapeutic intervention and inhibitory peptides."

Hi Neil,Here's my feedback for your article, structured in the format of the following 4 questions from our Wiki Edu training:

1. What does he article do well?

The additional information added to the article is very methodical and neutral. It's perfect for the neturality required in a Wikipedia article. YOu describe the core of GP41 in a systematic way, by explaining sequentially how certain areas become exposed and help with fusion to the host cell. our citation is also ideally placed, not interrupting the flow of sentences. You also cite from a scientific article, which is a valid, peer-reviewed source of information, perfect for Wikipedia. I also enjoy that you included a section on structure. This makes perfect sense for a transmembrane protein whose structure is pivotal to how it can be chemically targeted. Neutrality and balance are not an issue here because your added explanations are descriptions of the protein.

2. What parts of the article could be improved?

Starting with the structure section: while this section is informative and necessary, I think you could be more careful in how it gels together with other sections. Organizationally, it might be better to move the "Gene and PTM" section to after the structure, since logically this is how I'd understand the protein: its structure, how that structure is modified, and finally the use of its structure and modification in functioning. It's also a little unclear transitioning between those 2 sections. In the gene section it is stated that gp120 and gp41 and cleaved, but in your structure section you say they noncovalently bind each other. Which is it? If it's both, just make it clear when each thing happens so readers aren't confused Also, you may want to consider adding more links to words like "heptad repeat" and "disulfide bond". Same advice for your additions to the "function" section. You also say the disulfide bond is "important" without mentioning it later. Either say why it's important, or don't say it's important, otherwise this leaves a gap in information. It's good that you cite this sentence, though, so people can refer to it for more details.

3. What's the most important thing the author can do to improve the article?

Your edits are already pretty content-heavy, neutral, balanced, and well-cited. I'd say the main thing is leaving nothing ambiguous (see terms above) or unexplained, or if you choose not to explain, refer readers to the source where they can learn more. Also, consider changing the overall structure of the article as mentioned above. As well, if you have time, consider adding to the "gene and PTM" section, as it's a little bare, and is not cited. You might include research on how its expression is regulated in the virus.

4. Did you find anything about this article that could be applied to your own?

You briefly mention how the conformation of gp120 changes the expose binding sites for other coreceptors. This would be similar to the sequential model (my article) in that the sequential model describes how conformational changes to a protein modulate its affinity for substrates. However, I think the sequential model mostly applies to those that have multiple binding sites for the same substrate, resulting in sigmoidal binding curves. So I guess there isn't really anything directly applicable - but I learned a lot from reading your article!

Ruwan23 (talk) 20:31, 3 March 2017 (UTC)Ruwan Thilakaratne

Gp41

Potential References:

More Review Articles at PubMed, search: "(gp41 AND Review[ptyp] AND full text[sb] AND "last 5 years"[PDat])"

Outline of Proposed Changes

1. Overall - The section will be expanded to be at least as comprehensive as the gp120 Wikipedia page.
2. Chemistry - The current wikipedia page talks about a "cascade of conformational changes" but does not go into detail about them, so the "Function" section can be expanded to include those details using the equine analogous protein gp45. Additionally, the fusion and activation processes can also be expanded upon. The structure of gp41 can also be described in detail using the following article: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4594655/ and https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4909398/.
3. As a drug target - There are a number of potential inhibitory pathways that can be included in this section.

Article Options

Immunoglobulin class switching

CD4

Gp41

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Natural killer cell

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Tumor antigen

PubMed Searches:

https://www.ncbi.nlm.nih.gov/pubmed/?term=immunoproteins+biochemistry (w/ review, human, and last year filters)