Akroshilina
Welcome!
editHello, Akroshilina, and welcome to Wikipedia! My name is Ian and I work with the Wiki Education Foundation; I help support students who are editing as part of a class assignment.
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If you have any questions, please don't hesitate to contact me on my talk page. Ian (Wiki Ed) (talk) 21:05, 31 May 2016 (UTC)
Feedback request
editHi Akroshilina. I got your ping, and I took a look at what you've been up to. Your sandbox draft is a good start, but it needs some work in terms of style.
The useful features of NgAgo for genome editing include the following. First, it has a low tolerance to guide–target mismatch. A single nucleotide mismatch at each position of the gDNA impaired the cleavage efficiency of NgAgo, and mismatches at three positions completely blocked cleavage in our experiments. Second, 5′ phosphorylated short ssDNAs are rare in mammalian cells, which minimizes the possibility of cellular oligonucleotides misguiding NgAgo. Third, NgAgo follows a 'one-guide-faithful' rule, that is, a guide can only be loaded when NgAgo protein is in the process of expression, and, once loaded, NgAgo cannot swap its gDNA with other free ssDNA at 37 °C.
When you say "useful features...include the following. First..." you're actually using a lot more words than you need, and that makes it harder to read. The key with encyclopedia writing is to get information across to the reader quickly. When you say "include the following" you add three words that add very little useful information to the sentence. Instead of that, you might want to try something more like:
The useful features ofNgAgo is useful for genome editing because of itsinclude the following. First, it has alow tolerance to guide–target mismatch. A single nucleotide mismatch at each position of the gDNA impaired the cleavage efficiency of NgAgo, and mismatches at three positions completely blocked cleavage in our experiments.SecondIn addition, 5′ phosphorylated short ssDNAs are rare in mammalian cells, which minimizes the possibility of cellular oligonucleotides misguiding NgAgo.Third,NgAgo follows a 'one-guide-faithful' rule, that is, a guide can only be loaded when NgAgo protein is in the process of expression, and, once loaded, NgAgo cannot swap its gDNA with other free ssDNA at 37 °C.
If you do that, you produce text that's easier to read and that has less of a step-by-step narrative and more of a whole one. Ian (Wiki Ed) (talk) 21:45, 1 June 2016 (UTC)
Looks good. Just make sure you include your source. Ian (Wiki Ed) (talk) 21:13, 7 June 2016 (UTC)