The Vg1 ribozyme is a manganese dependent RNA enzyme or ribozyme which is the smallest ribozyme to be identified. It was identified in the 3′ UTR of Xenopus Vg1 mRNA transcripts and mouse beta-actin mRNA.[1] This ribozyme was identified from in vitro studies that showed that the Vg1 mRNA was cleaved within the 3′ UTR in the absence of protein. Studying the Vg1 mRNA 3′UTR a manganese-dependent ribozyme was predicted to exist. This ribozyme was shown to be located adjacent to the polyadenylation site and in vitro studies showed that it catalyzes a first-order reaction where its mechanism of cleavage is similar to the manganese ribozyme present in Tetrahymena group I introns.[2]
| Manganese dependent ribozyme in Vg1 mRNA | |
|---|---|
 Secondary structure of the VG1 ribozyme. | |
| Identifiers | |
| Symbol | VG1 |
| Rfam | RF01865 |
| Other data | |
| RNA type | Ribozyme |
| Domain(s) | Xenopus |
| PDB structures | PDBe |
In vivo studies showed that this ribozyme is not functional with the cell.[1]
References
edit- ^ a b Kolev NG, Hartland EI, Huber PW (October 2008). "A manganese-dependent ribozyme in the 3′-untranslated region of Xenopus Vg1 mRNA". Nucleic Acids Res. 36 (17): 5530–5539. doi:10.1093/nar/gkn530. ISSN 0305-1048. PMC 2553595. PMID 18753150.
- ^ Dange V, Van Atta RB, Hecht SM (May 1990). "A Mn2(+)-dependent ribozyme". Science. 248 (4955): 585–588. Bibcode:1990Sci...248..585D. doi:10.1126/science.2185542. PMID 2185542.
External links
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