![](http://upload.wikimedia.org/wikipedia/commons/thumb/b/b4/Q-SORT2018_Edit-a-Thon_-_Training_Session.jpg/220px-Q-SORT2018_Edit-a-Thon_-_Training_Session.jpg)
The CHASSY Project ([1]) is a joint scientific project working on the topic of yeast-based biotechnology. To communicate the topics and results the project organizes a Wikipedia training and Edit-a-thon. The goal of this course is to edit and update descriptions in Wikipedia of concepts such as yeast biotechnology, CRISPR technologies and related topcs, where information gaps currently exist (see below). The event is organised by Achim Raschka (wikipedian from Germany, employee of Nova-Institut as consortium partner) with support from the University College Cork, Delft University of Technology, University of Frankfurt, Chalmers University of Technology and Institut national de la recherche agronomique (INRA).
The CHASSY Wikipedia Edit-a-thon is meant for anybody who uses Wikipedia and we invite scientist, researchers and Ph.D students interested in the addressed topics to participate.
Participants
editWhere and when
editThe Editathon will be organised as an online event running for several sessions. The participants start with a first session on creating an account and sign-up at this page, then starting with first editing and later on working together on dedicated articles linked to the topics of the project.
To discuss problems and other topics a set of meeting will be held via Zoom (or other software) at specific dates.
What did the Edit-a-thon's participants bring?
edit- Their laptops(and a chargers!)
- Their Wikipedia login details (same of them created a free account here)
- Their enthusiasm to help contribute to the world's greatest encyclopaedia
Wikipedia Policies
edit- Wikipedia:Five pillars
- Wikipedia:Core content policies
- Wikipedia:General notability guideline
- Wikipedia:Verifiability
- Wikipedia:Conflict of interest
- Wikipedia:Identifying reliable sources
- Wikipedia:No original research (Examples of Original Research)
- Wikipedia:Citing sources
- Wikipedia:Identifying and using primary sources
Quick Editing Tips
editTools and Other Resources
edit- Here is a short tutorial for getting started editing Wikipedia
- Wikipedia:Citing sources
- Wikipedia:Cheatsheet
- Wikipedia:Citation templates
- Wikipedia:Principles
- Wikipedia:Tutorial
- Wiki markup quick reference (PDF)
- Track Wikipedia Page Views
- Wikimedia Statistics
- Wiki Education Foundation
- Wiki Ed Foundation's online training modules
- Internet Archive Wayback Machine
- Last year's editing event at the Sumner school
Outcomes
editThe main focus to work on will be the english Wikipedia, but since CHASSY is an international project the students and scientists may also work on the wikis in their mother language (German, Dutch, French, Swedish, Irish Gaelic, Russian)
Article wishlist
editPages to be created ex-novo or expanded/improved:
- lead scientists (with photos)
- John P. Morrissey (professor at UCC, WikiData)
- Jean-Marc Daran (professor at TU Delft, WikiData)
- Jack Pronk (professor at TU Delft, WikiData)
- Eckhard Boles (professor at University Frankfurt, WikiData, Eckhard Boles (German Wikipedia))
- Jens Nielsen (professor at Chalmers, WikiData (?))
- Jean-Marc Nicaud (Research Director CNRS, INRA ([1], WikiData)
- yeast species
- main topics of the project
- Biotechnology, Industrial biotechnology (no article), History of biotechnology, Yeast biotechnology[2]
- Yeast expression platform
- Genome editing, CRISPR, CRISPR/Cas9, Cas9[3]
- crRNA, CRISPR/Cpf1, Cpf1[4]
- Mandelic acid, Octanoic acid, Naringenin
- Malonyl-CoA, Acetyl-CoA
- Very long chain fatty acid (VLCFA), Docosanol, Fatty acid synthase (FAS)[5], Very-long-chain 3-oxoacyl-CoA synthase
- Fatty-acyl-CoA Synthase, FATP1, SLC27A1[6]
- Systems biology
- Expression cassette, Synthetic biology[7]
- Genome engineering, Adaptive laboratory evolution
- Biosensor[8]
- Bioinformatics, Genome-scale metabolic model, GECKO[9], Ribosome profiling[10]
- Enzyme kinetics, Flux balance analysis, Molecular crowding[9]
- Major facilitator superfamily, Ribosome profiling[11]
Pictures wishlist
edit- Photos of cultures from the yeats, molecular biology toolsets, others ...
- Photos of lead scientists
- ...
Results
editThe following results are made from this course:
- Eckhard Boles (German Wikipedia), Eckhard Boles
- John P. Morrissey (German Wikipedia), John P. Morrissey (biologist)
- Category:Irish microbiologists (John as first one)
- Ribosome profiling[10]
- Ogataea
- ...
References and project publications
edit- ^ CHASSY entry on European Commission website; Project Website
- ^ Robert Mans, Jean-Marc Daran, Jack T. Pronk: Under pressure: evolutionary engineering of yeast strains for improved performance in fuels and chemicals production. Current Opinion in Biotechnology 50, April 2018, Pages 47-56. doi:10.1016/j.copbio.2017.10.011.
- ^ Hannes Juergens, Javier A. Varela, Arthur R. Goter de Vries, Thomas Perli, Veronica Gast, Nikola Y. Gyurchev, Arun S. Rajkumar, Robert Mans,Jack T. Pronk, John P. Morrissey, Jean-Marc Daran: Genome editing in Kluyveromyces and Ogataea yeasts using a broad-host-range Cas9gRNA co-expression plasmid. FEMS Yeast Research 18, 2018, foy012. doi:10.1093/femsyr/foy012.
- ^ Michal A. Swiat, Sofia Dashko, Maxime den Ridder, Melanie Wijsman, John van der Oost, Jean-Marc Daran, Pascale Daran-Lapujade: FnCpf1: a novel and efficient genome editing tool for Saccharomyces cerevisiae. Nucleic Acids Research 45 (21), 2017; P. 12585–12598. doi:10.1093/nar/gkx1007.
- ^ Tao Yu, Yongjin J. Zhou, Leonie Wenning, Quanli Liu, Anastasia Krivoruchko, Verena Siewers, Jens Nielsen & Florian David: Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals. Nature Communications 8, 2017, article number 15587. doi:10.1038/ncomms15587.
- ^ Yating Hua, Zhiwei Zhua, Jens Nielsen, Verena Siewers: Heterologous transporter expression for improved fatty alcohol secretion in yeast. Metabolic Engineering 45, January 2018; Pages 51-58. doi:10.1016/j.ymben.2017.11.008.
- ^ M. Larroude, T. Rossignol, J.-M. Nicaud, R. Ledesma-Amaro: Synthetic biology tools for engineering Yarrowia lipolytica. Biotechnology Advances 36 (8), December 2018; Pages 2150-2164. doi:10.1016/j.biotechadv.2018.10.004.
- ^ Leonie Baumann , Arun S. Rajkumar, John P. Morrissey, Eckhard Boles, Mislav Oreb: A Yeast-Based Biosensor for Screening of Short- and Medium-Chain Fatty Acid Production. ACS Synthetic Biology 7 (11), 2018; pp 2640–2646. doi:10.1021/acssynbio.8b00309.
- ^ a b Benjamín J. Sánchez, Cheng Zhang, Avlant Nilsson,Petri‐Jaan Lahtvee, Eduard J. Kerkhoven, Jens Nielsen: Improving the phenotype predictions of a yeast genome‐scale metabolic model by incorporating enzymatic constraints. Molecular Systems Biology 13, 2017; p. 935. doi:10.15252/msb.20167411.
- ^ a b Michel AM, Baranov PV (September 2013). "Ribosome profiling: a Hi-Def monitor for protein synthesis at the genome-wide scale". Wiley Interdisciplinary Reviews: RNA. 4 (5): 473–90. doi:10.1002/wrna.1172. PMC 3823065. PMID 23696005.
- ^ Javier A. Varela, Martina Puricelli, Noemi Montini and John P. Morrissey: Expansion and Diversification of MFS Transporters in Kluyveromycesmarxianus. Frontiers in Microbiology 9, 2019; article :3330. doi:10.3389/fmicb.2018.03330.