In enzymology, a xyloglucan-specific endo-beta-1,4-glucanase (EC 3.2.1.151) is an enzyme that catalyzes the chemical reaction
| Xyloglucan-specific endo-beta-1,4-glucanase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 3.2.1.151 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- xyloglucan + H2O xyloglucan oligosaccharides
Thus, the two substrates of this enzyme are xyloglucan and H2O, whereas its product is xyloglucan oligosaccharides.
This enzyme belongs to the family of hydrolases, specifically those glycosidases that hydrolyse O- and S-glycosyl compounds. The systematic name of this enzyme class is [(1->6)-alpha-D-xylo]-(1->4)-beta-D-glucan glucanohydrolase. Other names in common use include XEG, xyloglucan endo-beta-1,4-glucanase, xyloglucanase, xyloglucanendohydrolase, XH, and 1,4-beta-D-glucan glucanohydrolase.
Family 12 was first identified in plant pathogens by discovery in Phytophthora spp.
Structural studies
editAs of late 2007, 15 structures have been solved for this class of enzymes, with PDB accession codes 2CN2, 2CN3, 2E0P, 2E4T, 2EEX, 2EJ1, 2EO7, 2EQD, 2JEM, 2JEN, 2JEP, 2JEQ, 2UWA, 2UWB, and 2UWC.
References
editFurther reading
edit- Pauly M, Andersen LN, Kauppinen S, Kofod LV, York WS, Albersheim P, Darvill A (January 1999). "A xyloglucan-specific endo-beta-1,4-glucanase from Aspergillus aculeatus: expression cloning in yeast, purification and characterization of the recombinant enzyme". Glycobiology. 9 (1): 93–100. doi:10.1093/glycob/9.1.93. PMID 9884411.
- Grishutin SG, Gusakov AV, Markov AV, Ustinov BB, Semenova MV, Sinitsyn AP (November 2004). "Specific xyloglucanases as a new class of polysaccharide-degrading enzymes". Biochimica et Biophysica Acta (BBA) - General Subjects. 1674 (3): 268–81. doi:10.1016/j.bbagen.2004.07.001. PMID 15541296.
- Costanzo S, Ospina-Giraldo MD, Deahl KL, Baker CJ, Jones RW (October 2006). "Gene duplication event in family 12 glycosyl hydrolase from Phytophthora spp". Fungal Genetics and Biology. 43 (10): 707–14. doi:10.1016/j.fgb.2006.04.006. PMID 16784880.
