NotI

NotI is a restriction enzyme isolated from the bacterium Nocardia otitidis.^[1]

Recognition sequence

Palindromic recognition sequence of NotI:^[1]

5'-GCGGCCGC-3'
3'-CGCCGGCG-5'

The ends generated by NotI digest:

5'---GC     GGCCGC---3'
3'---CGCCGG     CG---5'

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v t e Restriction modification system: restriction enzyme
Basic Concept	Isoschizomer Neoschizomer Isocaudomer
Recognition sequence 4bp	TaqI Sau3A AluI* HaeIII* DpnII NlaIII HpaII
Recognition sequence 5bp	EcoRII FokI
Recognition sequence 6bp	AaaI BglII PovII* EcoRV* EcoRI BamHI HindIII XbaI XhoI SacI PstI NdeI
Recognition sequence 8bp	NotI
Lists	A Ba–Bc Bd–Bp Bsa–Bso Bsp–Bss Bst–Bv C-D E-F G-K L-N O-R S T-Z
* means cleavage produces blunt ends