Pseudomonas sRNA P16 is a ncRNA that was predicted using bioinformatic tools in the genome of the opportunistic pathogen Pseudomonas aeruginosa and its expression verified by northern blot analysis.[1] P16 sRNA appears to be conserved across several Pseudomonas species and is consistently located downstream of a predicted TatD deoxyribonuclease gene. P16 has a predicted Rho independent terminator at the 3′ end but the function of P16 is unknown.[1]
| Pseudomonas sRNA P16 | |
|---|---|
 Predicted secondary structure and sequence conservation of P16 | |
| Identifiers | |
| Symbol | P16 |
| Rfam | RF00628 |
| Other data | |
| RNA type | Gene |
| Domain(s) | Bacteria |
| SO | SO:0000655 |
| PDB structures | PDBe |
It has been shown that this sRNA is transcribed from an RpoS-dependent promoter under positive, probably indirect GacA control in two Pseudomonas species. It was renamed RgsA (for regulation by GacA and stress).[2] RpoS mRNA expression is repressed by RgsA during the exponential phase. The Hfq RNA chaperone is required for the repression.[3]
See also
editReferences
edit- ^ a b Livny J, Brencic A, Lory S, Waldor MK (2006). "Identification of 17 Pseudomonas aeruginosa sRNAs and prediction of sRNA-encoding genes in 10 diverse pathogens using the bioinformatic tool sRNAPredict2". Nucleic Acids Res. 34 (12): 3484–3493. doi:10.1093/nar/gkl453. PMC 1524904. PMID 16870723.
- ^ González, Nicolas; Heeb, Stephan; Valverde, Claudio; Kay, Elisabeth; Reimmann, Cornelia; Junier, Thomas; Haas, Dieter (2008-04-13). "Genome-wide search reveals a novel GacA-regulated small RNA in Pseudomonas species". BMC Genomics. 9: 167. doi:10.1186/1471-2164-9-167. ISSN 1471-2164. PMC 2375449. PMID 18405392.
- ^ Lu, Pei; Wang, Yifei; Hu, Yangbo; Chen, Shiyun (April 2018). "RgsA, an RpoS-dependent sRNA, negatively regulates rpoS expression in Pseudomonas aeruginosa". Microbiology. 164 (4): 716–724. doi:10.1099/mic.0.000632. ISSN 1465-2080. PMID 29473822.
External links
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