SFI1

Sfi1 homolog, spindle assembly associated (yeast) is a protein that in humans is encoded by the SFI1 gene. It localizes to the centriole, and its S. pombe ortholog has been shown to be involved in spindle pole body duplication. SFI1 forms a complex with centrin 2.

SFI1
Available structures PDB Ortholog search: PDBe RCSB List of PDB id codes 2K2I
Identifiers
Aliases	SFI1, PISD, PPP1R139, hSfi1p, SFI1 centrin binding protein
External IDs	OMIM: 612765; MGI: 1926137; HomoloGene: 12707; GeneCards: SFI1; OMA:SFI1 - orthologs
Gene location (Human) Chr. Chromosome 22 (human) Band 22q12.2 Start 31,488,688 bp End 31,618,588 bp
Gene location (Mouse) Chr. Chromosome 11 (mouse) Band 11|11 A1 Start 3,081,850 bp End 3,143,463 bp
RNA expression pattern Bgee Human Mouse (ortholog) Top expressed in right hemisphere of cerebellum right uterine tube granulocyte left testis anterior pituitary right testis sural nerve spleen right lobe of thyroid gland left lobe of thyroid gland Top expressed in bronchus trachea lobar bronchus spermatocyte superior frontal gyrus mesothelium of visceral serous pericardium primary visual cortex yolk sac Rostral migratory stream right ventricle More reference expression data BioGPS n/a
Gene ontology Molecular function phosphatase binding protein binding Cellular component cytoplasm centriole cytosol centrosome cytoskeleton Biological process negative regulation of phosphatase activity G2/M transition of mitotic cell cycle ciliary basal body-plasma membrane docking regulation of G2/M transition of mitotic cell cycle Sources:Amigo / QuickGO
Orthologs Species Human Mouse Entrez 9814 78887 Ensembl ENSG00000198089 ENSMUSG00000023764 UniProt A8K8P3 Q3UZY0 RefSeq (mRNA) NM_001007467 NM_001258325 NM_001258326 NM_001258327 NM_014775 NM_030207 NM_001363222 NM_001363223 NM_001363224 RefSeq (protein) NP_001007468 NP_001245254 NP_001245255 NP_001245256 NP_055590 NP_084483 NP_001350151 NP_001350152 NP_001350153 Location (UCSC) Chr 22: 31.49 – 31.62 Mb Chr 11: 3.08 – 3.14 Mb PubMed search
Wikidata
View/Edit Human View/Edit Mouse

Further reading

• Martinez-Sanz J, Kateb F, Assairi L, Blouquit Y, Bodenhausen G, Abergel D, Mouawad L, Craescu CT (January 2010). "Structure, dynamics and thermodynamics of the human centrin 2/hSfi1 complex". Journal of Molecular Biology. 395 (1): 191–204. doi:10.1016/j.jmb.2009.10.041. PMID 19857500.
• Mayor T, Stierhof YD, Tanaka K, Fry AM, Nigg EA (November 2000). "The centrosomal protein C-Nap1 is required for cell cycle-regulated centrosome cohesion". The Journal of Cell Biology. 151 (4): 837–46. doi:10.1083/jcb.151.4.837. PMC 2169446. PMID 11076968.
• Petretti C, Savoian M, Montembault E, Glover DM, Prigent C, Giet R (April 2006). "The PITSLRE/CDK11p58 protein kinase promotes centrosome maturation and bipolar spindle formation". EMBO Reports. 7 (4): 418–24. doi:10.1038/sj.embor.7400639. PMC 1456919. PMID 16462731.
• Casenghi M, Meraldi P, Weinhart U, Duncan PI, Körner R, Nigg EA (July 2003). "Polo-like kinase 1 regulates Nlp, a centrosome protein involved in microtubule nucleation". Developmental Cell. 5 (1): 113–25. doi:10.1016/S1534-5807(03)00193-X. PMID 12852856.
• Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI (December 2000). "Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry". The EMBO Journal. 19 (23): 6569–81. doi:10.1093/emboj/19.23.6569. PMC 305846. PMID 11101529.
• Kilmartin JV (September 2003). "Sfi1p has conserved centrin-binding sites and an essential function in budding yeast spindle pole body duplication". The Journal of Cell Biology. 162 (7): 1211–21. doi:10.1083/jcb.200307064. PMC 2173958. PMID 14504268.
• Hendrickx A, Beullens M, Ceulemans H, Den Abt T, Van Eynde A, Nicolaescu E, Lesage B, Bollen M (April 2009). "Docking motif-guided mapping of the interactome of protein phosphatase-1". Chemistry & Biology. 16 (4): 365–71. doi:10.1016/j.chembiol.2009.02.012. PMID 19389623.
• Takahashi M, Yamagiwa A, Nishimura T, Mukai H, Ono Y (September 2002). "Centrosomal proteins CG-NAP and kendrin provide microtubule nucleation sites by anchoring gamma-tubulin ring complex". Molecular Biology of the Cell. 13 (9): 3235–45. doi:10.1091/mbc.E02-02-0112. PMC 124155. PMID 12221128.
• Golsteyn RM, Mundt KE, Fry AM, Nigg EA (June 1995). "Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle function". The Journal of Cell Biology. 129 (6): 1617–28. doi:10.1083/jcb.129.6.1617. PMC 2291169. PMID 7790358.
• Rüthnick D, Vitale J, Neuner A, Schiebel E (March 2021). "The N-terminus of Sfi1 and yeast centrin Cdc31 provide the assembly site for a new spindle pole body". J Cell Biol. 220 (3). doi:10.1083/jcb.202004196. PMC 7852455. PMID 33523111.

External links

• Overview of all the structural information available in the PDB for UniProt: A8K8P3 (Protein SFI1 homolog) at the PDBe-KB.