tRNA (cytidine34-2'-O)-methyltransferase (EC 2.1.1.207, yibK (gene), methyltransferase yibK, TrmL, tRNA methyltransferase L, tRNA (cytidine34/5-carboxymethylaminomethyluridine34-2'-O)-methyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (cytidine34/5-carboxymethylaminomethyluridine34-2'-O)-methyltransferase.[1] This enzyme catalyses the following chemical reaction
| tRNA (cytidine34-2'-O)-methyltransferase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 2.1.1.207 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| |||||||||
- (1) S-adenosyl-L-methionine + cytidine34 in tRNA S-adenosyl-L-homocysteine + 2'-O-methylcytidine34 in tRNA
- (2) S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine34 in tRNALeu S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine34 in tRNALeu
The enzyme from Escherichia coli catalyses the 2'-O-methylation of cytidine or 5-carboxymethylaminomethyluridine at the wobble position at nucleotide 34 in tRNALeuCmAA and tRNALeucmnm5UmAA.
References
edit- ^ Benítez-Páez A, Villarroya M, Douthwaite S, Gabaldón T, Armengod ME (November 2010). "YibK is the 2'-O-methyltransferase TrmL that modifies the wobble nucleotide in Escherichia coli tRNA(Leu) isoacceptors". RNA. 16 (11): 2131–43. doi:10.1261/rna.2245910. PMC 2957053. PMID 20855540.
External links
edit- TRNA+(cytidine34-2'-O)-methyltransferase at the U.S. National Library of Medicine Medical Subject Headings (MeSH)
